bio-rad伯乐 Mini-PROTEAN 3 电泳系统

功能灵活的Mini–PROTEAN 3 电泳槽既可使用 Ready Gel 预制胶，也可使用手灌胶。 它是目前开发的相关产品中方便的手灌胶操作系 统。Mini–PROTEAN 3 电泳系统省去了冗长的组装程 序，简化了灌胶和电泳过程。Mini–PROTEAN 3 电泳 槽可用于：

• 一小时内完成小型胶电泳
• 一天内完成双向凝胶电泳
• 用于探索型的课题研究
• 筛选新样品
• 评估样品制备条件

• 封边垫条永远地固定在长玻板上，保证玻板对齐，防止漏胶
• 凸轮卡锁的制胶框操作简单^*，在任何平面上都能对齐玻板
• 特殊的塑料电泳梳^*不会抑制凝胶聚合反应，制胶过程中，内置的脊可避免的空气接触，保证均一的凝胶聚合
• 标有厚度和孔数的玻板和电泳梳便于识别
• 平行排列的灌胶架*能同时看到正在灌制的两块凝胶，弹簧杠杆设计使得软橡胶衬垫产生良好的密封性
• 含封边垫条的长玻板加厚，使得玻板不宜破碎。

• 的上样引导装置^**，防止泳道的遗漏上样或重复上样
• 电泳槽可同时进行2 块凝胶电泳，或使用塑模档板进行1 块凝胶电泳

模块式设计可使Mini–PROTEAN 3 电泳槽有多种用途。每个模块可与Mini–PROTEAN 3 的缓冲液槽和盖匹配， 形成一个完整的电泳槽。有以下4 个模块可选择：

• Mini–PROTEAN 3 电泳槽，适合手灌胶或预制PAGE 和SDS–PAGE 胶
• 小型Trans–Blot 电泳槽，适合western 印迹杂交；
• 422 型电洗脱仪，用于电洗脱样品条带或整个泳道以进行探索研究；
• Mini–PROTEAN 2–D 电泳槽，用于双向应用中的优异向IEF 管式凝胶；

更多信息，请索取资料＃ 2378。

 

 

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		电泳梳厚度
孔数或孔类型	孔宽度	0.5 mm	0.75 mm	1.0 mm	1.5 mm
5	12.70 mm	—	70 µl	105 µl	160 µl
9	5.08 mm	—	33 µl	44 µl	66 µl
10	5.08 mm	22 µl	33 µl	44 µl	66 µl
15	3.35 mm	13 µl	20 µl	26 µl	40 µl
IPG	76.20 mm	—	—	420 µl	730 µl
Prep/2–D
Reference well	3.10 mm	—	13 µl	17 µl	30 µl
Sample well	71.70 mm	—	310 µl	400 µl	680 µl

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只需3 个简单的步骤就可将Mini–PROTEAN II电泳槽改装成Mini–PROTEAN 3电泳槽：

1. 保留Mini–PROTEAN II 的槽和盖。
2. 定购Mini–PROTEAN II 升组合套件（货号#165–3375）。
3. 定购所需的制胶模块。

可从预设配置中选择电泳系统，定购货号为#165–3301（10 孔、0.75 mm）或165–3322 （10 孔、1.0 mm）。 如要装配你自己的电泳系统，你可以购买Mini–PROTEAN 3 电泳模块（货号#165–3302）并从订货信息中另外选 择一种制胶模块。如果你只使用ReadyGel 预制胶，Mini–PROTEAN 3 电泳模块能满足你的需要。

凝胶数	1 或2
预制胶	Ready Gel 预制胶
手灌胶	Mini-PROTEAN 3 长玻板灌制
玻板尺寸(W x L)
短板	10.1 x 7.3 cm
间隔板	10.1 x 8.2 cm
凝胶大小(W x L)	手灌胶：8.3 x 7.3 cm; 预制胶： 8.6 x 6.8 cm
典型上层缓冲液体积	120 ml
典型下层缓冲液体积	180 ml
典型SDS-PAGE电泳时间	45 min ( 200 V 恒压)
建议电源	PowerPac Basic 或 PowerPac HC
大小(W x L x H)	12 x 16 x 18 cm
重量	2.0 kg

目录 #	描述
Mini-PROTEAN 3 Cell
165-3301	Mini-PROTEAN 3 Electrophoresis Cell, 10-well, 0.75 mm thickness; complete system, includes 2 combs, 5 sets of glass plates, casting stand, casting clamp assembly, sample loading guide, electrophoresis module (electrode assembly, clamping frame, tank, lid with power cables, mini cell buffer dam)
165-3302	Mini-PROTEAN 3 Electrophoresis Module, for Ready Gel precast gel applications, includes electrode assembly, clamping frame, tank, lid with power cables, mini cell buffer dam
Systems
165-2959	Mini-PROTEAN 3 Electrophoresis Cell/Mini Trans-Blot Electrophoretic Transfer Cell, 10-well, 0.75 mm thickness; complete system includes 2 combs, 5 sets of glass plates, casting stand, casting clamp assembly, sample loading guide, electrophoresis module (electrode assembly, clamping frame, tank, lid with power cables, mini cell buffer dam), 2 gel holder cassettes, 4 fiber pads, modular electrode assembly, Bio-Ice cooling unit, lower buffer chamber, lid with cables, instructions
165-3317	Mini-PROTEAN 3 Electrophoresis Cell and Mini Trans-Blot Module, 10-well, 0.75 mm thickness; complete system, includes 2 combs, 5 sets of glass plates, casting stand, casting clamp assembly, sample loading guide, electrophoresis module (electrode assembly, clamping frame, tank, lid with power cables, mini cell buffer dam), Mini Trans-Blot module
165-3318	Mini-PROTEAN 3 Electrophoresis Module and Mini Trans-Blot Module, includes electrode assembly, clamping frame, tank, lid with power cables, mini cell buffer dam, Mini Trans-Blot module
165-3319	Mini-PROTEAN 3 Cell and PowerPac Basic Power Supply, 10-well, 0.75 mm thickness; complete system, includes 2 combs, 5 sets of glass plates, casting stand, casting clamp assembly, sample loading guide, electrophoresis module (electrode assembly, clamping frame, tank, lid with power cables, mini cell buffer dam), 100–120/220–240 V power supply includes power cord, instructions
165-3321	Mini-PROTEAN 3 Cell and Single-Row AnyGel Stand, includes 10-well, 0.75 mm thickness; complete system, includes 2 combs, 5 sets of glass plates, casting stand, casting clamp assembly, sample loading guide, electrophoresis module (electrode assembly, clamping frame, tank, lid with power cables, mini cell buffer dam), single-row AnyGel stand holds 1 PROTEAN gel, 2 Criterion gels, or 3 Ready Gel mini gels; includes instructions
165-3323	Mini-PROTEAN 3 Electrophoresis Cell, Mini Trans-Blot Module, and PowerPac Basic Power Supply, 10-well, 1.0 mm thickness; complete system, includes 2 combs, 5 sets of glass plates, casting stand, casting clamp assembly, sample loading guide, electrophoresis module (electrode assembly, clamping frame, tank, lid with power cables, mini cell buffer dam), Mini Trans-Blot module, power supply
165-3324	Mini-PROTEAN 3 Electrophoresis Module, Mini Trans-Blot Module, and PowerPac Basic Power Supply, 10-well, 1.0 mm thickness; complete system, includes 2 combs, 5 sets of glass plates, casting stand, casting clamp assembly, sample loading guide, electrophoresis module (electrode assembly, clamping frame, tank, lid with power cables, mini cell buffer dam), Mini Trans-Blot module, power supply
170-3989	Mini Trans-Blot Cell and PowerPac Basic Power Supply, includes 2 gel holder cassettes, 4 fiber pads, modular electrode assembly, Bio-Ice cooling unit, lower buffer tank, lid with cables, power supply, power cord, instructions
Accessories
165-3320	Gel Releasers, 5
165-4131	AnyGel Stand, single-row, holds 1 PROTEAN gel, 2 Criterion gels, or 3 Ready Gel mini gels; includes instructions
165-4132	Replacement Clamps, 2
165-5131	AnyGel Stand, 6-row, holds 6 PROTEAN gels, 12 Criterion gels, or 18 ReadyGel mini gels; includes instructions
Casting Modules
165-3325	Casting Module, 10-well, 0.5 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide
165-3326	Casting Module, 15-well, 0.5 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide
165-3327	Casting Module, 5-well, 0.75 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide
165-3328	Casting Module, 9-well, 0.75 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide
165-3329	Casting Module, 10-well, 0.75 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide
165-3330	Casting Module, 15-well, 0.75 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide
165-3331	Casting Module, prep/2-D well, 0.75 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates
165-3332	Casting Module, 5-well, 1.0 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide
165-3333	Casting Module, 9-well, 1.0 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide
165-3334	Casting Module, 10-well, 1.0 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide
165-3335	Casting Module, 15-well, 1.0 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide
165-3336	Casting Module, prep/2-D well, 1.0 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates
165-3337	Casting Module, IPG well, 1.0 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates
165-3338	Casting Module, 5-well, 1.5 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide
165-3339	Casting Module, 9-well, 1.5 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide
165-3340	Casting Module, 10-well, 1.5 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide
165-3341	Casting Module, 15-well, 1.5 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide
165-3342	Casting Module, prep/2-D well, 1.5 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates
165-3343	Casting Module, IPG well, 1.5 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates